Here are a list of protocols from our lab. Some of the protocols were developed in my previous labs (and might be optimized by our current lab).
How to avoid dauer-associated effects on longevity under reduced Insulin/IGF-1 signaling in the nematode Caenorhabditis elegans
Teuscher AC, Statzer C, Pantasis S, Bordoli MR, Ewald CY.
Methods Mol Biol. 2019;1944:169-188. doi: 10.1007/978-1-4939-9095-5_13.