Here are a list of protocols from our lab. Some of the protocols were developed in my previous labs (and might be optimized by our current lab).
Also check our contributions on Github for details: https://github.com/Ewaldlab-LSD
How to avoid dauer-associated effects on longevity under reduced Insulin/IGF-1 signaling in the nematode Caenorhabditis elegans
Here is the protocol of how to make rapamycin plates for lifespan experiments.
This protocol was used in:
- TOR Signaling and Rapamycin Influence Longevity by Regulating SKN-1/Nrf and DAF-16/FoxO. DOI: 10.1016/j.cmet.2012.04.007 PMCID: PMC3348514
- Dauer-independent insulin/IGF-1-signalling Implicates Collagen Remodelling in Longevity. DOI: 10.1038/nature14021 PMCID: PMC4352135
SKN-1::GFP microscopyOvercoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis elegans
Teuscher AC, Statzer C, Pantasis S, Bordoli MR, Ewald CY.
Methods Mol Biol. 2019;1944:169-188. doi: 10.1007/978-1-4939-9095-5_13.
The C. elegans matrisome
The in-silico characterization of the Caenorhabditis elegans matrisome and proposal of a novel collagen classification
Alina C. Teuscher, Elisabeth Jongsma, Martin N.Davis, Cyril Statzer, Jan M.Gebauer, Alexandra Naba, Collin Y. Ewald
Browse our C. elegans collagen database (CeColDB): http://CeColDB.permalink.cc/
Use our Matrisome Annotator on your datasets: http://ce-matrisome-annotator.permalink.cc/
Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
#Collagen #crosslinking is a major pathology during #aging, however, it is difficult to assess. @AnneHaefke shows how to use the "Tail Tendon Break Time for the Assessment of #Aging and Longitudinal #Healthspan in #Mice". @bioprotocolbyte #ewaldlab @eth