Protocols
Here are a list of protocols from our lab. Some of the protocols were developed in my previous labs (and might be optimized by our current lab).
Also check our contributions on Github for details: https://github.com/Ewaldlab-LSD
How to avoid dauer-associated effects on longevity under reduced Insulin/IGF-1 signaling in the nematode Caenorhabditis elegans
Rapamycin Plates
Here is the protocol of how to make rapamycin plates for lifespan experiments.
This protocol was used in:
- TOR Signaling and Rapamycin Influence Longevity by Regulating SKN-1/Nrf and DAF-16/FoxO. DOI: 10.1016/j.cmet.2012.04.007 PMCID: PMC3348514
- Dauer-independent insulin/IGF-1-signalling Implicates Collagen Remodelling in Longevity. DOI: 10.1038/nature14021 PMCID: PMC4352135
SKN-1::GFP microscopy
Overcoming Autofluorescence to Assess GFP Expression During Normal Physiology and Aging in Caenorhabditis eleganshttps://bio-protocol.org/bio101/e2940
Full-text
Oxidative Stress Assays (arsenite and tBHP) in Caenorhabditis elegans
If you are looking for a detailed protocol of how to assess oxidative tolerance using C. elegans, please see our bio-protocol.org article.
Teuscher AC, Statzer C, Pantasis S, Bordoli MR, Ewald CY.
Methods Mol Biol. 2019;1944:169-188. doi: 10.1007/978-1-4939-9095-5_13.
The C. elegans matrisome
The in-silico characterization of the Caenorhabditis elegans matrisome and proposal of a novel collagen classification
Matrix Biol. Plus. 2019 https://doi.org/10.1016/j.mbplus.2018.11.001
Alina C. Teuscher, Elisabeth Jongsma, Martin N.Davis, Cyril Statzer, Jan M.Gebauer, Alexandra Naba, Collin Y. Ewald
Browse our C. elegans collagen database (CeColDB): http://CeColDB.permalink.cc/
Use our Matrisome Annotator on your datasets: http://ce-matrisome-annotator.permalink.cc/
Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
#Collagen #crosslinking is a major pathology during #aging, however, it is difficult to assess. @AnneHaefke shows how to use the "Tail Tendon Break Time for the Assessment of #Aging and Longitudinal #Healthspan in #Mice". @bioprotocolbyte #ewaldlab @eth
https://bio-protocol.org/e3833
© 2016